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Biomol GmbH ac-devd-afc fluorogenic substrate
Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+fluorogenic+substrate/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
Average 90 stars, based on 1 article reviews
ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Local Tissue Expression of the Cell Death Ligand, Fas Ligand, Plays a Central Role in the Development of Extrapulmonary Acute Lung Injury
Article Snippet: Fifty micrograms of tissue protein in 50 2L of buffer was added to 50 2L of caspase 3 reaction buffer (containing 100 mM HEPES, 2% sucrose, 0.2% CHAPS, 10 mM dithiothreitol [all from Sigma], and 100 2M Ac-DEVD-AFC fluorogenic substrate [Biomol, Plymouth Meeting, Pa]).

Article Title: Antifibrogenic role of the cannabinoid receptor CB2 in the liver.
Article Snippet: ORIS JULIEN,* PASCALE GRENARD,* FATIMA TEIXEIRA–CLERC,* EANNE TRAN VAN NHIEU,* LIYING LI,* MELIHA KARSAK, ANDREAS ZIMMER, RIANE MALLAT,* and SOPHIE LOTERSZTAJN* INSERM, U581, Créteil, F-94010 France; Université Paris XII-Val de Marne, Créteil, F-94010, Créteil France; AP-HP, Hôpital Henri Mondor, épartement de Pathologie, Créteil, F-94010 France; AP-HP, Hôpital Henri Mondor, Service d’Hépatologie et de Gastroentérologie, Créteil, -94010 France; and University of Bonn, Department of Psychiatry, Bonn, Germany

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Article Title: hnRNPs Relocalize to the Cytoplasm following Infection with Vesicular Stomatitis Virus
Article Snippet: Immediately prior to assaying the caspase activity, 50 μl of activation buffer (100 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)], 0.2 mM EDTA, and 20% glycerol) containing Ac-DEVD-AFC fluorogenic substrate (BioMol) and 1 mM dithiothreitol were added to the remaining 40 μl of lysed cells.

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Fluorescence:

Article Title: Local Tissue Expression of the Cell Death Ligand, Fas Ligand, Plays a Central Role in the Development of Extrapulmonary Acute Lung Injury
Article Snippet: Fifty micrograms of tissue protein in 50 2L of buffer was added to 50 2L of caspase 3 reaction buffer (containing 100 mM HEPES, 2% sucrose, 0.2% CHAPS, 10 mM dithiothreitol [all from Sigma], and 100 2M Ac-DEVD-AFC fluorogenic substrate [Biomol, Plymouth Meeting, Pa]).

Article Title: Antifibrogenic role of the cannabinoid receptor CB2 in the liver.
Article Snippet: ORIS JULIEN,* PASCALE GRENARD,* FATIMA TEIXEIRA–CLERC,* EANNE TRAN VAN NHIEU,* LIYING LI,* MELIHA KARSAK, ANDREAS ZIMMER, RIANE MALLAT,* and SOPHIE LOTERSZTAJN* INSERM, U581, Créteil, F-94010 France; Université Paris XII-Val de Marne, Créteil, F-94010, Créteil France; AP-HP, Hôpital Henri Mondor, épartement de Pathologie, Créteil, F-94010 France; AP-HP, Hôpital Henri Mondor, Service d’Hépatologie et de Gastroentérologie, Créteil, -94010 France; and University of Bonn, Department of Psychiatry, Bonn, Germany

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Article Title: hnRNPs Relocalize to the Cytoplasm following Infection with Vesicular Stomatitis Virus
Article Snippet: Immediately prior to assaying the caspase activity, 50 μl of activation buffer (100 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)], 0.2 mM EDTA, and 20% glycerol) containing Ac-DEVD-AFC fluorogenic substrate (BioMol) and 1 mM dithiothreitol were added to the remaining 40 μl of lysed cells.

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Activity Assay:

Article Title: Local Tissue Expression of the Cell Death Ligand, Fas Ligand, Plays a Central Role in the Development of Extrapulmonary Acute Lung Injury
Article Snippet: Fifty micrograms of tissue protein in 50 2L of buffer was added to 50 2L of caspase 3 reaction buffer (containing 100 mM HEPES, 2% sucrose, 0.2% CHAPS, 10 mM dithiothreitol [all from Sigma], and 100 2M Ac-DEVD-AFC fluorogenic substrate [Biomol, Plymouth Meeting, Pa]).

Article Title: Antifibrogenic role of the cannabinoid receptor CB2 in the liver.
Article Snippet: ORIS JULIEN,* PASCALE GRENARD,* FATIMA TEIXEIRA–CLERC,* EANNE TRAN VAN NHIEU,* LIYING LI,* MELIHA KARSAK, ANDREAS ZIMMER, RIANE MALLAT,* and SOPHIE LOTERSZTAJN* INSERM, U581, Créteil, F-94010 France; Université Paris XII-Val de Marne, Créteil, F-94010, Créteil France; AP-HP, Hôpital Henri Mondor, épartement de Pathologie, Créteil, F-94010 France; AP-HP, Hôpital Henri Mondor, Service d’Hépatologie et de Gastroentérologie, Créteil, -94010 France; and University of Bonn, Department of Psychiatry, Bonn, Germany

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Article Title: hnRNPs Relocalize to the Cytoplasm following Infection with Vesicular Stomatitis Virus
Article Snippet: Immediately prior to assaying the caspase activity, 50 μl of activation buffer (100 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)], 0.2 mM EDTA, and 20% glycerol) containing Ac-DEVD-AFC fluorogenic substrate (BioMol) and 1 mM dithiothreitol were added to the remaining 40 μl of lysed cells.

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Activation Assay:

Article Title: Local Tissue Expression of the Cell Death Ligand, Fas Ligand, Plays a Central Role in the Development of Extrapulmonary Acute Lung Injury
Article Snippet: Fifty micrograms of tissue protein in 50 2L of buffer was added to 50 2L of caspase 3 reaction buffer (containing 100 mM HEPES, 2% sucrose, 0.2% CHAPS, 10 mM dithiothreitol [all from Sigma], and 100 2M Ac-DEVD-AFC fluorogenic substrate [Biomol, Plymouth Meeting, Pa]).

Article Title: Antifibrogenic role of the cannabinoid receptor CB2 in the liver.
Article Snippet: ORIS JULIEN,* PASCALE GRENARD,* FATIMA TEIXEIRA–CLERC,* EANNE TRAN VAN NHIEU,* LIYING LI,* MELIHA KARSAK, ANDREAS ZIMMER, RIANE MALLAT,* and SOPHIE LOTERSZTAJN* INSERM, U581, Créteil, F-94010 France; Université Paris XII-Val de Marne, Créteil, F-94010, Créteil France; AP-HP, Hôpital Henri Mondor, épartement de Pathologie, Créteil, F-94010 France; AP-HP, Hôpital Henri Mondor, Service d’Hépatologie et de Gastroentérologie, Créteil, -94010 France; and University of Bonn, Department of Psychiatry, Bonn, Germany

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Article Title: hnRNPs Relocalize to the Cytoplasm following Infection with Vesicular Stomatitis Virus
Article Snippet: Immediately prior to assaying the caspase activity, 50 μl of activation buffer (100 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)], 0.2 mM EDTA, and 20% glycerol) containing Ac-DEVD-AFC fluorogenic substrate (BioMol) and 1 mM dithiothreitol were added to the remaining 40 μl of lysed cells.

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).



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